Fig. 3

CPZ-mediated ER stress induces an autophagic response – western blot. Determination, by means of western blot (images, left) and their relative quantification (histograms, right) of the autophagy markers LC3 II and p62 in control (CTL) and CPZ-treated cells, either in the presence (grey columns) or absence (white columns) of the ER stress inhibitor 4-PBA, in control (CTL) or CPZ-treated T98G, U-87 MG and U-251 MG anchorage-dependent GBM cells (A), TS#1, TS#83 and TS#163 neurospheres (B) and RPE-1 non-cancer cells (C). β-actin determination has been used for relative quantification. When present, statistical significance is indicated (*p<0.05; **p<0.01; ***p<0.001)