Fig. 2

GAS6-AS1 promotes leukemia cell proliferation in vitro and vivo. A CCK-8 assays detecting cell proliferation of HL-60, U937 and Kasumi-1 cells following transfection-mediated GAS6-AS1 knockdown or overexpression. B Colony-forming assays detecting cell proliferation of HL-60, U937 and Kasumi-1 cells. C EdU assays examining cell proliferation after transfection. D, E, F Scramble vector or sh-GAS6-AS1 was transfected into U937 cells, which were subcutaneously injected in mice, and the tumors were obtained at day 21 and weighed (n = 5). G, H, I The tumor sections were under Hematoxylin and Eosin (H&E) staining and immunohistochemical staining using antibodies against Ki-67. Data were depicted as mean ± s.e.m., *P < 0.05