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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Therapeutic inhibition of GAS6-AS1/YBX1/MYC axis suppresses cell propagation and disease progression of acute myeloid leukemia

Fig. 6

GAS6-AS1 facilitates leukemia cell proliferation via YBX1-mediated transactivation of MYC. A Co-IP and Western blot detecting endogenous interaction between YBX1 and MYC in U937 cells. IgG, negative control. B Co-IP and Western blot showing the interaction between YBX1 with MYC in U937 cells stably transfected with empty vector (mock), GAS6-AS1, scramble shRNA (sh-Scb), or sh-GAS6-AS1. C, D Dual-luciferase assay indicating the activity of MYC in AML cells stably transfected with sh-Scb, or sh-GAS6-AS1#1 and those co-transfected with YBX1 (n = 4). E, F Quantification of colony formation assay of leukemic cells transfected with a series of vectors. Data were depicted as mean ± s.e.m., *P < 0.05. G Gene Set Enrichment Analysis (GSEA) demonstrating the gene sets of regulation of apoptosis and the MAPK pathway were enriched in GAS6-AS1-downexpressing cells. H Pathway enrichment analysis using the Kyoto Encyclopedia of Genes and Genomes (KEGG)

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