Fig. 1

ZIP10 is required for osteosarcoma proliferation and chemoresistance. a qRT-PCR results of zinc transporter ZIP/SLC39 family expression in Saos-2 and 143B osteosarcoma (OS) cell lines with or without cisplatin treatment. b WB results of ZIP10 expression in 2 cell lines. c Representative IHC staining images of OS specimens with (post) or without (pre) chemotherapy. Scale bar, 100 μm. d ZIP10 expression level of OS specimens. e An analysis was performed to examine ZIP10 expression in human OS cell lines and normal mesenchymal stem cells (MSCs). f Left, CCK-8 assay was performed to determine the cell proliferation of negative control (NC) and stable ZIP10-knockdown (shZIP10) cell lines; right, the efficiency of shRNA targeting ZIP10 was determined by WB analysis. g Colony formation activity of 143B cells with/without cisplatin (Cis) treatment. h Photographed xenograft tumors (top), average tumor volume (bottom, left) and average tumor weight (bottom, right) of NC and shZIP20 xenografts with/without cisplatin treatment. Scale bar = 1 cm. N = 6 mice per group. Data are means ± SEM; *P < 0.05, **P < 0.01