Fig. 2

Knockdown of ZIP10 inhibits PI3K/AKT-mediated cell proliferation and chemoresistance in osteosarcoma. a KEGG pathway annotations of differentially expressed genes. The bar plot presents the enrichment scores (−log10[P value]) of the top 10 significantly enriched KEGG pathways. b Gene array analysis of NC and shZIP10 Saos-2 cells. Downregulated genes associated with the PI3K/AKT signaling pathway are shown. Rows represent individual genes; columns represent individual samples. Pseudocolors indicate transcript levels below (green), equal to (yellow) or above (red) the mean. The scale represents the relative gene expression (fold change between 0.1 and 1.1). c The expression levels of AKT, p-AKT, ERK, p-ERK, JNK and p-JNK were analyzed by WB using 143B and Saos-2 cells with/without ZIP10 knockdown. d ZIP10 knockdown (shZIP10), ZIP10 overexpression (oeZIP10), AKT activation (SC79) and AKT inhibitor (GSK690693) were applied to 143B cells treated with DMSO (Con) or cisplatin (Cis) for 72 h. Flow cytometry was conducted to determine apoptosis. e The whole-cell extract of 143B cells treated with cisplatin for 72 h was subjected to WB analysis. f EdU incorporation assay determination of the proliferation of 143B cells. Scale bar, 10 μm. Data are means ± SEM; *P < 0.05, **P < 0.01