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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: TMPRSS4 promotes cancer stem–like properties in prostate cancer cells through upregulation of SOX2 by SLUG and TWIST1

Fig. 2

TMPRSS4 promotes resistance to anoikis and drugs. A To induce anoikis, transfected PC3 cells were seeded in 96-well plates with an Ultra-Low Attachment Surface at a density of 1.5 × 104 cells/well and grown for up to 7 days. Cell viability was determined by colorimetric WST assay. B Transfected PC3 cells were incubated for 48 h with 0.5% bovine serum albumin under suspension culture conditions, and then stained with annexin V and PI for flow cytometry. The proportion of stained cells was determined to calculate the percentage of apoptotic cells. C Transfected PC3 cells (5 × 106 cells/mouse) were intravenously injected into nude mice (n = 4). Twenty-four hours after injection, lungs were removed to extract total DNA. Real-time qPCR analysis was performed on human PTGER2 with total DNA extracted from lungs. The amounts of human genomic DNA initially present in the qPCR reaction tube were extrapolated (Upper) from the standard curve generated by real-time qPCR performed on human total DNA extracted from PC3 parental cells mixed with mouse total DNA from lungs of nude mice (Lower). D Transfected PC3 cells were treated with anti-cancer drugs for 72 h. Cell viability was determined by colorimetric WST assay. DMSO (0.1%) was used as a vehicle. Values represent mean ± SD. *P < 0.05; ***P < 0.001

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