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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: TMPRSS4 promotes cancer stem–like properties in prostate cancer cells through upregulation of SOX2 by SLUG and TWIST1

Fig. 4

SLUG and TWIST1 are required for TMPRSS4-mediated ALDH activity and anoikis resistance. A–C PC3 cells were co-transfected with a TMPRSS4 expression vector and siRNA specific to SLUG or TWIST1 for 48 h. A Transfected cells were harvested and then subjected to ALDH assay. B Transfected cells were incubated under suspension culture conditions and then cell viability was determined by colorimetric WST assay. C Transfected cells were lysed for immunoblot analysis. D, E PC3 cells were co-transfected with TMPRSS4 expression vector and siRNA specific to BMI1 or SOX2 for 48 h. Transfected cells were subjected to ALDH assay (D) or lysed for immunoblot analysis (E). Anti-myc antibody was used to detect myc-tagged TMPRSS4. Densitometric quantification was performed on the immunoblots using GAPDH as a loading control. The mean relative density from three independent experiments is shown under the immunoblots (C, E). Values represent mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001 compared with vector + control siRNA; §§P < 0.01; §§§P < 0.001 compared with TMPRSS4 + control siRNA

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