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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: CircRNA-DOPEY2 enhances the chemosensitivity of esophageal cancer cells by inhibiting CPEB4-mediated Mcl-1 translation

Fig. 4

cDOPEY2 functions by suppressing CPEB4. A K-M plot showing the relationship between cDOPEY2 levels and patient overall survival (OS) in a TCGA data set. B Representative IHC staining images of CPEB4 in ESCC tissues and nontumorous tissues (NTs). Scale bars: 100 μm. C Western blotting was performed to detect CPEB4 in the indicated cells with cDOPEY2 overexpression or knockdown. D The time-course viability of the indicated cells after treatment with cisplatin (10 µg/mL) was determined by the CCK-8 method. E Clonogenic assay of the indicated cells treated with increasing doses of cisplatin. F The apoptotic rate of the indicated cells 24 h after treatment with cisplatin (10 µg/mL) was determined by Annexin V-FITC/PI FACS analysis. G Relative caspase-3 activity in the indicated ESCC cells 24 h after cisplatin treatment at a dose of 10 µg/mL. H Western blotting was performed to detect CPEB4 and Mcl-1 in the indicated cells. Data are presented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. P values were determined by one-way ANOVA with Tukey’s post hoc test (D, E, F and G) and log-rank test (A)

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