Fig. 1From: Upregulation of ERK-EGR1-heparanase axis by HDAC inhibitors provides targets for rational therapeutic intervention in synovial sarcomaHDACi treatment induces variable death response in SS cells. a Caspase 3 activation induced by SAHA was analyzed after 72 h by Western blotting in SYO-1, CME-1 and Yamato-SS cells, or after 72 h and 96 h in MoJo cells. Arrows indicate fragments of activated caspase 3. Actin, tubulin and GAPDH show protein loading. Caspase 3 enzyme activity was measured in SYO-1 and CME-1 cells after 72 h-treatment by a colorimetric assay. Data are reported as arbitrary units ± SE from two independent experiments. b SYO-1 and CME-1 cells, exposed to 10 nM FK228 for 18 h and 24 h, respectively, were subjected to apoptosis detection by TUNEL assay. Data are reported as mean % of TUNEL-positive cells ± SE from two independent experiments and representative dot plots of FACS analysis are shown. *P < 0.05Back to article page