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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Upregulation of ERK-EGR1-heparanase axis by HDAC inhibitors provides targets for rational therapeutic intervention in synovial sarcoma

Fig. 2

FK228 treatment induces early EGR1 and PTEN upregulation and ERK activation followed by AKT inhibition in SS cells. Cells, exposed to FK228 for the indicated times, were processed for Western blot analysis with the indicated antibodies. Acetylation of histones H3 (K12) and H4 (K27) is shown as marker of HDAC inhibition. Cleavage of caspase 3 evidenced cell death in SYO-1 and CME-1. Arrows indicate fragments of activated caspase 3. Anti-actin, −tubulin, −vinculin and -GAPDH blots show protein loading. Numbers represent the intensity of relevant bands normalized to the respective loading controls

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