Fig. 5
SF3B1 expression and consequences of its modulation in PDAC CSCs. A, B SF3B1 mRNA levels (normalized to ACTB expression) in adherent (ADH; A)- vs. sphere (SPH; S)-derived PDX PDAC cells. The SF3B1 mRNA levels are grouped in A or individual in B for the PDX-derived cell set (A6L, 215, 253 and 354) (n = 2). C. Representative scheme of PDAC PDX-derived cell line generation and treatment with Pladienolide-B. D, E Quantification of flow cytometric analysis of the percentage of autofluorescent (Fluo) and CD133-positive cells in the indicated PDX-derived in vitro cultures treated with or without (vehicle) 1 nM Pladienolide-B (n = 3). F Log2 mRNA expressions levels of genes implicated in stemness normalized to HPRT expression (n = 4). Data shown are mean ± SEM. Asterisks indicate significant differences (*p < 0.05; **p < 0.01; ***p < 0.001)