Fig. 7
Pladienolide-B reduces malignancy features of PDAC cells and CSCs in vivo. A-B Fold-change ± SEM of MIAPaCa-2 and A6L h2b-mCherry cells in zebrafish embryos, calculated by measuring the area and the fluorescence intensity. Cells were injected after 24 h of Pladienolide-B (Pd) treatment. Changes in Pd-treated cells were compared to control at indicated days post injection (dpi). C Representative images of Control- and Pd-treated A6L-zebrafish xenografts at indicated dpi. D Tumor volume (mm3) of MIAPaCa-2-xenografts at indicated days after xenografting. Red arrow indicates Pladienolide-B injection. G1, control-treated mice, G2, Pd-treated mice. E MIAPaCa-2 xenograft tumor volumes, expressed as % ratio, extracted from Control- (n = 4) and Pd-treated (n = 5) mice at time of euthanasia (d49 after xenografting). F Pictures of paired Control- and Pd-treated tumors. Asterisks indicate significant differences (*p < 0.05; **p < 0.01; ***p < 0.001)