Fig. 8

Effects of FAK inhibition on its nuclear interactome. A PPI networks of the nuclear FAK interactors. B Representative immunoblot of nuclear extracts immunoprecipitated with antibody against FAK and then immunoblotted with FAK, HDAC1, and HDAC2 antibodies in HCC cells silenced or not for FAK and in MOCK (upper panels); representative immunoblot of nuclear extracts immunoprecipitated with antibody against FAK and then immunoblotted with pTyr397FAK, HDAC1 and HDAC2 antibodies in NT or TAE226 > SOR HCC cells, and in MOCK (lower panels). The experiments were repeated in duplicate (C) Quantitative imaging of immunofluorescence for pTyr397FAK, β-Catenin, HDAC1 and HDAC2 represented as fold change of mean fluorescence intensity in HepG2 and Huh7 cells treated vs. NT. Data were analysed by 2-tailed Student’s t test. ***p < 0.001 vs. NT. D H3K27ac levels measured by AlphaLISA assay and represented as fold change in HepG2 and Huh7 cells treated vs. NT. E Graphical abstract reporting the interaction of FAK with NuRD complex