Fig. 1

LINC01050 expression is up-regulated in gastric cancer tissues and is associated with a poor prognosis. a The top 15 dysregulated lncRNAs in gastric cancer from TCGA data. b Relative expression of LINC01050 in GC tissues compared with noncancerous tissues, based on TCGA data. P < 0.001. c Patients with high expression of LINC01050 showed reduced survival times compared to a low expression of LINC01050 (p = 0.014). d qRT-PCR analysis of LINC01050 expression in a normal gastric epithelium cell line (GES-1) and gastric cancer cell lines (AGS, KATO III, and BGC823). Data are presented as mean ± SD (n = 3). ^**P < 0.01. e qRT-PCR analysis of LINC01050 expression in the nuclear and cytoplasmic fractions from KATO III cells. GAPDH was used as the cytoplasmic control, and U6 as the nucleus control. The data are represented as the mean ± SD (n = 3). f RNA-FISH detection of LINC01050 (red) in GC cell lines (KATO III, BGC823, AGS, and HGC-27) and GES-1 cells. The nuclei were counterstained using DAPI (blue). FISH, fluorescence in situ hybridization. Scale bar = 10 μm