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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: C-Myc-activated long non-coding RNA LINC01050 promotes gastric cancer growth and metastasis by sponging miR-7161-3p to regulate SPZ1 expression

Fig. 2

LINC01050 is a direct transcriptional target of c-Myc. a Luciferase activity of LINC01050 promoter constructs with a deletion encompassing the c-Myc binding sites in HEK293T cells transfected with pIRES2-vector or pIRES2-c-Myc. Overexpression of c-Myc in HEK293T cells was confirmed by Western blotting. Data are presented as mean ± SD (n = 3).*P < 0.05; **P < 0.01; ***P < 0.001. ns, not significant. b ChIP analysis of c-Myc enrichment at the LINC01050 promoter in KATO III cells. The data are represented as the mean ± SD. ***P < 0.001. c Western blot analysis of c-Myc protein expression in KATO III cells transfected with pIRES2-vector or pIRES2-c-Myc. Data are presented as mean ± SD (n = 3). **P < 0.01. d qRT-PCR analysis of LINC01050 expression in KATO III cells transfected with pIRES2-vector or pIRES2-c-Myc. Data are presented as mean ± SD (n = 3). **P < 0.01. e Overexpression of c-Myc promoted KATO III cell growth as revealed by CCK8 assays. Data are presented as mean ± SD (n = 3). *P < 0.05. f Overexpression of c-Myc in KATO III cells promoted plate colony formation. Data are presented as mean ± SD (n = 3). *P < 0.05. g Western blot analysis of c-Myc expression in KATO III cells transfected with si-NC or si-c-Myc. Data are presented as mean ± SD (n = 3). *P < 0.05. h qRT-PCR analysis of LINC01050 expression in KATO III cells transfected with si-NC or si-c-Myc. Data are presented as mean ± SD (n = 3). **P < 0.01. i Downregulation of c-Myc inhibited KATO III cell growth as revealed by the CCK8 assays. **P < 0.01. j Downregulation of c-Myc in KATO III cell inhibited plate colony formation. Data are presented as mean ± SD (n = 3). **P < 0.01

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