Fig. 3

LINC01050 overexpression promotes cell proliferation, metastasis, and EMT in vitro and tumor growth in vivo. a qRT-PCR analysis of LINC01050 expression in KATO III cells transduced with pLVX-vector or pLVX-LINC01050. Data are presented as mean ± SD (n = 3). ^**P < 0.01. b-c Cell proliferation of KATO III cells transduced with pLVX-vector or pLVX-LINC01050 as determined by CCK8 (b) and EdU assays (c). Data are presented as mean ± SD (n = 3). ^*P < 0.05. Scale bar = 50 μm. d Colony-forming capabilities of KATO III cells transfected with pLVX-vector or pLVX-LINC01050, as determined by plate colony-formation assays. Data are presented as mean ± SD (n = 3). ^*P < 0.05. e Migration and invasion abilities of KATO III cells transduced with pLVX-vector or pLVX-LINC01050, as assessed by Transwell assays. Data are presented as mean ± SD (n = 3). ^**P < 0.01. f Western blot analysis of EMT-related proteins (E-cadherin and vimentin) in KATO III cells transduced with pLVX-vector or pLVX-LINC01050. GAPDH was used as an internal control. Data are presented as mean ± SD (n = 3). ^*P < 0.05. g Growth curves of tumors from KATO III cells transduced with pLVX-vector or pLVX-LINC01050 in tumor-bearing nude mice. Data are presented as mean ± SD (n = 5). ^*P < 0.05. h Tumor weights from KATO III cells transduced with pLVX-vector or pLVX-LINC01050 in tumor-bearing nude mice (right panel, n = 5). ^*P < 0.05. The data are presented as the mean ± SD. Three representative images of the tumors from the nude mice are shown (left panel, n = 5). i Immunohistochemistry to detect the proliferation markers Ki-67 and PTEN in tumor tissue sections. Scale bar = 50 μm