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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: C-Myc-activated long non-coding RNA LINC01050 promotes gastric cancer growth and metastasis by sponging miR-7161-3p to regulate SPZ1 expression

Fig. 5

LINC01050 knockdown inhibits gastric cancer cell migration and invasion in vitro and metastasis in vivo. a Migration and invasion abilities of tBGC823 and KATO III cells transfected with si-NC (negative control), si-LINC01050#1, or si-LINC01050#2, as assessed by Transwell assays. The data are presented as the mean ± SD. **P < 0.01. Scale bar = 100 μm. b Western blot analysis of EMT-related protein expression (E-cadherin and vimentin) in BGC823 and KATO III cells transfected with si-NC (negative control), si-LINC01050#1, or si-LINC01050#2. GAPDH was used as an internal control. Data are presented as mean ± SD (n = 3). *P < 0.05, **P < 0.01. c Statistical quantification of lung metastatic nodules (n = 5) produced after BGC823 cells transduced with lentiviral shNC or shLINC01050 were injected into nude mice via the tail vein. The data are represented as the mean ± SD. *P < 0.05. d Representative photographs showing the macroscopic appearance of lung metastases. e Body weights of mice were recorded after a tail vein injection of the BGC823 cells transduced with lentiviral shNC or shLINC01050. Data are presented as mean ± SD (n = 5).*P < 0.05

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