Fig. 5

LINC01050 knockdown inhibits gastric cancer cell migration and invasion in vitro and metastasis in vivo. a Migration and invasion abilities of BGC823 and KATO III cells transfected with si-NC (negative control), si-LINC01050#1, or si-LINC01050#2, as assessed by Transwell assays. The data are presented as the mean ± SD. ^**P < 0.01. Scale bar = 100 μm. b Western blot analysis of EMT-related protein expression (E-cadherin and vimentin) in BGC823 and KATO III cells transfected with si-NC (negative control), si-LINC01050#1, or si-LINC01050#2. GAPDH was used as an internal control. Data are presented as mean ± SD (n = 3). ^*P < 0.05, ^**P < 0.01. c Statistical quantification of lung metastatic nodules (n = 5) produced after BGC823 cells transduced with lentiviral shNC or shLINC01050 were injected into nude mice via the tail vein. The data are represented as the mean ± SD. ^*P < 0.05. d Representative photographs showing the macroscopic appearance of lung metastases. e Body weights of mice were recorded after a tail vein injection of the BGC823 cells transduced with lentiviral shNC or shLINC01050. Data are presented as mean ± SD (n = 5).^*P < 0.05