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Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: C-Myc-activated long non-coding RNA LINC01050 promotes gastric cancer growth and metastasis by sponging miR-7161-3p to regulate SPZ1 expression

Fig. 7

LINC01050 promotes gastric cancer cell proliferation, migration, invasion, and EMT by regulating the miR-7161-3p/SPZ1 axis. a qRT-PCR analysis of SPZ1 mRNA expression in KATO III cells transfected with the control shRNA (sh-NC) or sh-LINC01050. **P < 0.01. b Western blot analysis of SPZ1 protein level in KATO III cells stably expressing the sh-NC or sh-LINC01050. **P < 0.01. c qRT-PCR analysis of SPZ1 mRNA expression in KATO III stable cell line with the pLVX vector or overexpressing pLVX-LINC01050. **P < 0.01. d Western blot analysis of SPZ1 protein level in KATO III cells transduced with the pLVX vector or pLVX-LINC01050. **P < 0.01. e Western blot analysis of SPZ1 protein level in KATO III cells transfected with NC, miR-7161-3p mimics, pLVX-LINC01050 or pLVX-LINC01050 plus miR-7161-3p mimics. **P < 0.01, ***P < 0.001. f Growth curves of KATO III cells transfected with NC, miR-7161-3p mimics, pLVX-LINC01050 or pLVX-LINC01050 plus miR-7161-3p mimics, as revealed using CCK8 assays. *P < 0.05, **P < 0.01. g Migration and invasion capabilities of KATO III cells transfected with NC, miR-7161-3p mimics, pLVX-LINC01050 or pLVX-LINC01050 plus miR-7161-3p mimics, as revealed using Transwell assays. *P < 0.05, **P < 0.01. Scale bar = 100 μm. h Western blot analysis of EMT-related protein expression (E-cadherin and vimentin) in KATO III cells transfected with NC, miR-7161-3p mimics, pLVX-LINC01050 or pLVX-LINC01050 plus miR-7161-3p mimics. GAPDH protein was used as an internal control. *P < 0.05, **P < 0.01, ***P < 0.001. i Association of LINC01050 and SPZ1 expression levels. P < 0.0001

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