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Fig. 8 | Journal of Experimental & Clinical Cancer Research

Fig. 8

From: C-Myc-activated long non-coding RNA LINC01050 promotes gastric cancer growth and metastasis by sponging miR-7161-3p to regulate SPZ1 expression

Fig. 8

SPZ1 knockdown inhibits gastric cancer cell proliferation, migration, invasion, and EMT. a Relative expression of SPZ1 mRNA in gastric cancer tissues and normal tissues according to TCGA data (P < 0.001). b SPZ1 mRNA levels in KATO III cells transfected with si-NC or si-SPZ1, as determined by qRT-PCR. Data are presented as mean ± SD (n = 3). **P < 0.01. c SPZ1 protein expression in KATO III cells transfected with si-NC or si-SPZ1. Data are presented as mean ± SD (n = 3). **P < 0.01. d-e Proliferation of KATO III cells transfected with si-NC (negative control) or si-SPZ1, as determined using CCK8 (d) and EdU (e) assays. Data are presented as mean ± SD (n = 3). *P < 0.05, **P < 0.01. Scale bar = 50 μm. f Migration and invasion capabilities of KATO III cells transfected with si-NC or si-SPZ1, revealed using Transwell assays. The data are represented as the mean ± SD (n = 3). **P < 0.01. g Western blot analysis ofEMT-related protein expression (E-cadherin and vimentin) in KATO III cells transfected with si-NC or si-SPZ1. Data are presented as mean ± SD (n = 3). *P < 0.05. h A proposed model illustrating the regulatory role of c-Myc-activated lncRNA LINC01050 in promoting gastric cancer growth and metastasis by sponging miR-7161-3p to regulate SPZ1 expression

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