Fig. 2

SNHG17 promotes CRC proliferation and metastasis in vitro and in vivo. a The overexpression and knockdown efficiency of SNHG17 was detected by RT-qPCR after transfection with SNHG17 expression plasmid and three different SNHG17 siRNAs. b, c CCK-8 and colony formation assays were applied to detect the cell proliferation ability of HCT116 and LoVo cells after SNHG17 overexpression or knockdown. d, e Transwell assays were used to assess the migration and invasion abilities of HCT116 and LoVo cells after SNHG17 overexpression (d) or knockdown (e). f HCT116 cells stably overexpressing SNHG17 and SNHG17-depleted LoVo cells were subcutaneously injected into nude mice (n = 5) to evaluate the tumor formation ability in vivo. The tumor volume was measured every three days after tumors appeared, and the tumor weight was recorded after the mice were sacrificed. g HCT116 cells with SNHG17 overexpression and LoVo cells with SNHG17 knockdown were injected into the tail veins of nude mice (n = 5) to assess the metastasis ability of SNHG17. The number of lung nodules was counted after HE staining.