Fig. 4

SNHG17 upregulates FOSL2 by binding with miR-339-5p. a The subcellular localization of SNHG17 was detected by RT-qPCR and FISH in HCT116 and LoVo cell lines. b StarBase and RegRNA 2.0 were used to predict SNHG17-associated miRNAs. c The luciferase activity of SNHG17 after cotransfection with miRNAs was determined by dual luciferase assays. d The relative luciferase activity of cells cotransfected with SNHG17-WT or SNHG17-MUT and miR-339-5p mimic was determined in 293 T and HCT116 cells by luciferase reporter assays. e Cellular lysates from HCT116 and LoVo cells were used for RIP with Ago2 antibody and IgG antibody. The levels of SNHG17 and miR-339-5p were detected by RT-qPCR. f The StarBase and TargetScan databases were used to predict the targets of the SNHG17/miR-339-5p axis. g Luciferase reporter assays were used to determine the relative luciferase activity of FOSL2–3′ UTR-WT or FOSL2–3′ UTR-MUT after cotransfection with miR-339-5p mimics. h MiR-339-5p and pluc-FOSL2–3′ UTR-WT were cotransfected with SNHG17 to verify whether SNHG17 can function as a ceRNA of miR-339-5p. i The mRNA and protein levels of FOSL2 were determined in SNHG17-overexpressing or SNHG17-depleted CRC cells by RT-qPCR and western blotting. j The mRNA and protein levels of FOSL2 were determined in miR-339-5p-depleted or miR-339-5p-overexpressing CRC cells by RT-qPCR and western blotting. k-m CCK-8 (k), colony formation (l) and Transwell (m) assays were applied to detect the cell proliferation and migration abilities of HCT116 cells transfected with miR-339-5p and FOSL2 expression vector. n The mRNA expression levels of FOSL2 target genes were determined in HCT116 cells transfected with FOSL2 siRNA and/or SNHG17 expression plasmid by RT-qPCR.