Fig. 4From: Alternative splicing of NF-YA promotes prostate cancer aggressiveness and represents a new molecular marker for clinical stratification of patientsEffect of CRISPR-Cas9-mediated knock out of endogenous NF-YA in NF-YAs-overexpressing PC3 cells. A Western blot analysis of NF-YA isoforms in total cellular extracts from NF-YAs and NF-YAs-only (CRISPR-Cas9 edited clone #11 and #23) PC3 cells. Tubulin was used as loading control. B Optical microscopy images representative of the morphology of two different NF-YAs clones cultured as MTSs. The arrows indicate structures budding from MTS core. Scale bar 4X = 500 μm, scale bar 10X = 250 μm. C Rate of growth (%) of xenograft tumors measured for 5 consecutive weeks from s.c. inoculation of NF-YAs and NF-YAs-only edited PC3 cells into SCID Hairless Outbred (SHO®) mice. Data represent mean ± SEM (Two-way ANOVA with Holm-Sidak’s test n = 4)Back to article page