Fig. 7

Activity of NF-YAs and NF-YAl overexpression in cell migration and 3D invasion. A Percentage of cell migration of Empty, NF-YAl and NF-YAs cells measured by transwell assay. Data represent mean ± SEM (one-way ANOVA with Fisher’s LSD test: *p < 0.05, **p < 0.01, ns, not significant, n = 4). B Confluent cells were seeded into Ibidi wound-healing chambers and images were acquired at 0, 18, 20 and 22 h after the culture chambers were removed. (Left panel) Quantification of migration is shown as residual wound area compared to the initial gap, arbitrarily set at 100%. Data represent mean ± SEM (two-way ANOVA with Fisher’s LSD test: *p < 0.05, **p < 0.01 n = 6). (Right panel) Representative images of cell migration in wound-healing assay. C (Left panel) Representative phase contrast microscopy images of Empty, NF-YAl and NF-YAs MTSs. Spheroid invasion of MTSs included for 7 days in 1 mg/ml growth factors-enriched Matrigel. (Right panel) The histogram represents the percentage of invasion area vs total area of MTSs. Data represent mean ± SEM (unpaired t-test: *p < 0.05, ns, not significant, n = 3). D Phase contrast microscopy images representative of the invasive behaviour of MTSs when embedded into 5 mg/ml growth factors-reduced Matrigel surrounded by high-serum culture medium as chemoattractant for 7 and 11 days (n = 4). Scale bar = 500 μm