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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: PGRMC1-dependent lipophagy promotes ferroptosis in paclitaxel-tolerant persister cancer cells

Fig. 5

PGRMC1 promotes lipophagy via increased tubulin detyrosination. A Co-staining of lipid droplet (LD, green) and detyrosinated α-tubulin (red) in HN4 parental cells and PCC with or without PGRMC1 overexpression or inhibition. HN4 cells were transfected with control or PGRMC1 overexpression vector (vtr) or treated with 100 nM P4. HN4PCC were transfected with vector or shPGRMC1, or 20 μM AG205. The cells were also treated with DMSO, 10 μM erastin, or 10 μM erastin plus 20 μM parthenolide (PTN), a sesquiterpene lactone inhibiting the activity of tubulin carboxypeptidase (TCP) [24], for 24 h. Nuclei (blue) were stained with DAPI. Scale bar 5 μm. B An illustration showing the relation between tubulin detyrosination and lipophagy. C and D mRNA expression (C) and genomic DNA methylation levels (D) in HN4 parental cells and PCC with or without PGRMC1 overexpression or inhibition. Data are means and s.d. from three technical replicates. **P < 0.01 relative to vector control. E and F Immunoblotting in HN4 parental cells and PCC with or without PGRMC1 overexpression or inhibition and with DMSO or 10 μM erastin for 24 h. HN4 cells with control or PGRMC1 overexpression vector transfection were also transfected with scrambled or SIRT1 siRNA and treated with DMSO or 10 μM erastin for 24 h (F). G Co-staining for PGRMC1 (magenta), LC3-GFP (green), and nuclei (blue) in the cells treated with 10 μM EX527 (a selective SIRT1 inhibitor), 5 μM SRT1720 (a selective SIRT1 activator), 10 μM erastin, 20 μM PTN, or their combinations for 24 h. Scale bar 5 μm

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