Fig. 7

PGRMC1 expression increases ferroptosis sensitivity in vivo. A-E Representative images, tumor weights, and tumor volumes in HN4 parental cells and PCC with or without vector (vtr), PGRMC1 overexpression, or shPGRMC1 transfection, and vehicle (ctr) or SAS treatment. *P < 0.05, **P < 0.01 relative to vehicle control or between different groups. F and G Lipid droplet staining of tumors. Lipid droplets (green) were quantified using ImageJ and were normalized to HN4PCC vector control. Scale bar 100 μm. H Comparison of PGRMC1 mRNA expression between normal mucosa and tumor samples in the HNC patient cohort from TCGA datasets. t-test, P < 0.001. I Kaplan-Meier curves estimating overall survival (OS) according to patients with low and high expression levels of tumor PGRMC1 mRNA (cutoff = 34.57) from the TCGA datasets. Log-rank test, P < 0.001