Fig. 7

Zebrafish NSCLC BM xenograft models discriminated different chemosensitivities within 4 days. (A and B) NSCLC cell lines H1975 and A549 were involved. Transwell culture system was used to evaluate the effectiveness of osimertinib (1 µM) and gefitinib (2 µM) against cell invasion in vitro. Invaded cells were stained with crystal violet (0.5 %) and imaged after 24 h incubation. (C and D) Quantification of invaded cells in vitro. Colonies were quantified using Image Pro Plus. (E and F) NSCLC cell lines H1975 and A549 were involved. Tg (fli-1: EGFP) zebrafish embryos were injected about 100 cells (red fluorescence) into the PVs at 2 dpf and administered with osimertinib (1 µM) and gefitinib (13 µM) by intracardiac injection. (G and H) Quantification of cell brain metastasis at 4 dpi. Fold change of BM cell number was determined by dividing the number of BM cells in the same zebrafish at 4 dpi by the number of BM cells at 1 dpi. (ns) indicated statistical insignificance, (*) indicated statistical significance P < 0.05, (**) P < 0.01 and (***) P < 0.001