Fig. 2

Circ_0000658 is overexpressed in BCa tissues and cells and this overexpression is associated with poor prognosis of BCa patients. A The expression of circ_0000658 in BCa tissues and adjacent normal tissues determined with RT-qPCR (n = 50, *p < 0.001 vs. adjacent normal tissues); B The correlation between the expression of circ_0000658 and DFS and OS of patients with BCa analyzed with Kaplan-Meier analysis; C The location of circ_0000658 in cells determined with FISH assay; D The expression of circ_0000658 in BCa cells MGH-U3, T24, 5637, SW780 and normal bladder epithelial cell line SV-HUC-1 assessed with RT-qPCR (*p < 0.05 vs. SV-HUC-1 cells); E Silencing efficiency of circ_0000658 in T24 cells validated using RT-qPCR; F Silencing efficiency of circ_0000658 in 5637 cells validated using RT-qPCR (*p < 0.05 vs. sh-NC). All cell experiments were repeated three times