Fig. 4

Circ_0000658 competitively binds to miR-498 and reduces its expression. A Venn diagram of the intersection of downstream miRNAs targeted by circ_0000658 predicted using RegRNA 2.0 and circInteractome; B The binding site of circ_0000658 and miR-498 predicted using circInteractome; C Correlation analysis of circ_0000658 and miR-498 expression in BCa tissues; D miR-498 and circ_0000658 expression in T24 and 5637 cell lines determined with RT-qPCR (*p < 0.05 vs. oe-NC; #p < 0.05 vs. sh-NC); E Subcellular localization of circ_0000658 and miR-498 assayed with FISH; F Targeting relationship between circ_0000658 and miR-498 verified using dual-luciferase reporter gene assay (right panel) (*p < 0.05 vs. mimic NC); G The enrichment of circ_0000658 to miR-498 determined with RNA pull-down (*p < 0.05 vs. control); H The expression of miR-498 in BCa tissues and adjacent normal tissues (*p < 0.05 vs. adjacent normal tissues); I miR-498 expression in BCa tissues with or without lymphatic metastasis determined with RT-qPCR (*p < 0.05 vs. BCa tissues without lymphatic metastasis); J The relationship between BCa patients’ prognosis and miR-498 expression analyzed by the Kaplan-Meier method (*p < 0.05 vs. low miR-498 expression group). All cell experiments were repeated three times