Fig. 6

Circ_0000658 upregulates HMGA2 by downregulating miR-498 to augment EMT, proliferation, invasion and migration of BCa cells. Circ_0000658 was overexpressed or silenced in the presence or absence of miR-498 mimic or miR-498 inhibitor in T24 and 5637 cells. A The expression of circ_0000658, miR-498, and HMGA2 in T24 and 5637 cells determined with RT-qPCR; B Western blot analysis of protein expression of HMGA2 in T24 and 5637 cells; C The mRNA expression of EMT markers β-catenin, E-cadherin, N-cadherin, Slug, Snail, ZEB1 and Twist in T24 and 5637 cells determined with RT-qPCR; D The protein expression of EMT markers β-catenin, E-cadherin, N-cadherin, Slug, Snail, ZEB1 and Twist in T24 and 5637 cells determined with Western blot analysis; E The cell migration ability of T24 and 5637 cells measured with scratch test; F The cell proliferation of T24 and 5637 cells after circ_0000658 silencing determined with EdU assay; G The cell invasion of T24 and 5637 cells determined with Transwell assay. *p < 0.05 vs. oe-NC + mimic NC or sh-NC + inhibitor NC; #p < 0.05 vs. oe-circ_0000658 + mimic NC or sh-circ_0000658 + inhibitor NC. All cell experiments were repeated three times