Fig. 2

Other autophagy inhibitors did not enhance the anticancer activity of BKM120. A Western blot analysis of LC3B and p62 upon treatment with BKM120 and/or 10 μM Spautin-1 for 48 h. B Cell viability of SKOV-3 and MKN-1 cells after treatment with various concentrations of BKM120 alone or in combination with 10 μM Spautin-1 for 72 h. C The colony formation assay was used to measure the colony-forming abilities of SKOV-3 and MKN-1 cells treated with 10 μM Spautin-1 and/or BKM120. Colony quantification is graphed in (D). E SKOV-3 and MKN-1 cells were treated with BKM120 and/or 10 μM Spautin-1 for 48 h and subjected to measurement of total apoptotic cells. F Quantification of the apoptotic cell population. G SKOV-3 and MKN-1cells were treated with BKM120 alone or combined with 3-MA (0.5 mM) or Baf-A1 (1 nM) for 72 h and subjected to measurement of the relative cell viability. H Colony-forming abilities of SKOV-3 and MKN-1 cells treated with 3-MA (0.5 mM) or Baf-A1 (1 nM) alone or combined with BKM120. Colony quantification is graphed in (I). Graphs are normalized to 100% per treatment and are shown as the mean ± SD from three independent experiments; ns: p > 0.05