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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Hydroxychloroquine synergizes with the PI3K inhibitor BKM120 to exhibit antitumor efficacy independent of autophagy

Fig. 3

Silencing Atg5 did not affect the anticancer activity of BKM120. A Western blot analysis of p62, LC3 and Atg5 in SKOV-3 and MKN-1 cells after transfection with non-targeting siRNA or Atg5 siRNA. SKOV-3 and MKN-1 cells followed by treatment with BKM120 for 48 h. B, C Representative images of autophagosome puncta in SKOV-3 and MKN-1 cells transfected with non-targeting siRNA or Atg5 siRNA in combination with BKM120 treatment and quantification. Scale bar, 20 μm. D SKOV-3 and MKN-1 cells were transfected with or without Atg5 siRNA and were then treated with different concentrations of BKM120 for 72 h to detect cell viability by PrestoBlue assay. E Measurement of total apoptotic SKOV-3 and MKN-1 cells after transfection with non-targeting siRNA or Atg5 siRNA in combination with BKM120 treatment. F FACS quantification of the total apoptotic cell population. Graphs are normalized to 100% per treatment and are shown as the mean ± SD from three independent experiments; ns: p > 0.05; **p < 0.01

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