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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: BUB1 drives the occurrence and development of bladder cancer by mediating the STAT3 signaling pathway

Fig. 3

Molecular Interactions between STAT3 and BUB1. A. Schematic drawing of the domains and motifs of human BUB1 and STAT3. TPR: tetratricopeptide repeat. B. Co-IP and WB analysis of BUB1 and STAT3 proteins in total lysates obtained from T24, EJ and 5637 cells grown under serum-fed conditions. A mixture (1:1:1 ratio) of lysates from T24, EJ and 5637 cells was used in the IgG control sample. C. Co-IP and WB analysis of BUB1 and fragments of STAT3. D. Co-IP and WB analysis of STAT3 fragments and BUB1 in the input sample. E. Co-IP and WB analysis of the BUB1-P300 complex. F. Five thousand six hundred thirty-seven cells were transfected with the BUB1 wild-type vector for 24 h. Alexa Fluor 488 was used to stain BUB1 (green), Cy3 was used to stain STAT3 (red) and DAPI was used to stain cell nuclei (blue) for co-immunofluorescence (co-IF) analysis of the BUB1 and STAT3 proteins. G. Immunofluorescence staining (IF) of STAT3 (red) and BUB1 (green) in normal bladder and BCa tissues. Cell nuclei were visualized by DAPI staining

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