Fig. 4

Knockdown of BUB1 Attenuates the STAT3 Transcriptional Program. A. 5637 cell were treated with siBUB1. qRT–PCR analysis was performed to measure the mRNA levels of STAT3–induced target genes. The data are presented as the means ± SDs. B. The protein levels of BUB1, Ser727-STAT3, STAT3, NFATC2, LHX1 and GAPDH were measured by Western blotting in siBUB1-transfected 5637 and T24 cells. C. MTT experiments showed the IC50 value of BUB1 inhibitor 2OH-BNPP1. D. 5637 cell were treated with 10 μM 2OH-BNPP1. qRT–PCR analysis was performed to measure the mRNA levels of STAT3–induced target genes. The data are presented as the means ± SDs. E. The protein levels of BUB1, Ser727-STAT3, STAT3, NFATC2, LHX1 and GAPDH were measured by Western blotting in 10 μM 2OH-BNPP1-treated 5637 cell. F. T24 cells were treated with 10 μM 2OH-BNPP1. qRT–PCR analysis was performed to measure the mRNA levels of STAT3–induced target genes. The data are presented as the means ± SDs. G. The protein levels of BUB1, Ser727-STAT3, STAT3, NFATC2, LHX1 and GAPDH were measured by Western blotting in 10 μM 2OH-BNPP1-treated T24 cells