Fig. 7

BUB1 expression was related to the occurrence and development of bladder cancer. A. Flow chart of the method used to establish the mouse model of spontaneous bladder cancer. B. H&E staining of the bladders of mice treated with 0.1% BNN in different stages of disease progression. The first row shows the normal structure of the mouse bladder epithelium; the second row shows the structure of mouse bladder cancer in situ; the third row shows the structure of mouse bladder papillary carcinoma; and the fourth and fifth rows show the structure of mouse invasive bladder cancer invading muscle tissue and adipose tissue. C. Immunohistochemical staining of the bladders of mice treated with 0.1% BNN in different stages of disease. The first row shows the expression of BUB1 in normal mouse bladder epithelium; the second row shows the expression of BUB1 in mouse bladder cancer in situ; the third row shows the expression of BUB1 in mouse bladder papillary carcinoma; and the fourth row shows the expression of BUB1 in mouse invasive bladder cancer