Fig. 2

RHOA/ROCK mediate ROR2-induced tumor invasion. a pcDNA and pROR2 cells were characterized for the expression of non-canonical WNT signaling proteins by western blot. b Densitometric quantification of RHOA and ROCK2 in pcDNA and pROR2 cells normalized on HSP90 expression (mean ± SD, *p < 0.05, **p < 0.01, ns = not significant). c Active RHOA-GTP was measured in BT-474 cells by ELISA (mean ± SD, n = 4, *p < 0.05). d Invasion assay: MCF-7 pROR2 cells were treated for 96 h with the indicated concentration of Rhosin, a RHO inhibitor (mean ± SD, n = 3, *p < 0.05, **p < 0.01, ***p < 0.0001, ns = not significant). Significance was calculated with a one-way ANOVA with Dunnett’s multiple comparison test. e Cells were transfected with the indicated siRNAs (10 nM) and cell invasion was measured in Boyden chambers (mean ± SD, n = 3, *p < 0.05, **p < 0.01, ***p < 0.001, ns = not significant). Significance was calculated with a one-way ANOVA with Dunnett’s multiple comparison test