Fig. 2
tRF3008A inhibits colorectal cancer growth and migration in vitro: HCT116 cells were transfected with scrambled tRF mimetic control or tRF3008A mimetic, and HT29 cells were transfected with scrambled LNA control or tRF3008A-LNA. A&B Cell Counting Kit-8 and EdU assays were performed to evaluate cell proliferation. C Flow cytometry analysis of the apoptosis of HCT116 and HT29 cells with different expression levels of tRF3008A. D Cell migration and invasion abilities were evaluated by transwell migration and Matrigel invasion assays, respectively. E&F HCT116 cells or HT29 cells with different tRF3008A expression levels were subcutaneously implanted into nude mice, and then tumor growth was examined by measuring the tumor volume (every 3 days; n = 5). Line graph showing the tumor volume (mm3) from day 0 to day 21 after injection. Error bars represent the SD from 5 mice. For statistical analysis, Student’s t-test (two-sided, paired) was used. Three weeks after subcutaneous inoculation, the average tumor volumes in each group were calculated using the following formula: V (mm3) = (L × W2) × 0.5 (L: tumor length, W: width). G Four weeks after tail vein injection with HCT116 cells or HT29 cells with different tRF3008A expression levels, the luciferase signal intensities of mice were measured to identify metastasis foci (n = 10). H H&E staining for tissue morphology and immunohistochemistry staining for markers of proliferation (Ki67), apoptosis (CC3, cleaved caspase-3), and invasion (MMP9) in subcutaneous tumors. Data are represented as the means ± S.D. of at least three independent experiments. Statistical significance is measured using Student’s t test. Scare bar = 1000 μm. *: P < 0.05, **: P < 0.01, ***: P < 0.001