Fig. 4

tRF3008A inhibits the growth and EMT of CRC through the FOXK1/Wnt pathway. HCT116 cells were co-transfected with tRF3008A mimetic and sh-FOXK1 in the presence or absence of a Wnt pathway activator (Wnt agonist 1); HT29 cells were co-transfected with tRF3008A-LNA and OE-FOXK1 in the presence or absence of a Wnt pathway inhibitor (IWR-1-endo). A Western blot analysis of the levels of proteins related to the Wnt/β-catenin signaling pathway (C-JUN, c-Myc and CCND1) and EMT (Vimentin, snail and E-cadherin). β-Actin was used as a loading control. B Proliferation assessed using the Cell Counting Kit-8 assay. C&D EdU assays were performed to evaluate cell proliferative ability. E Active caspase-3 was analyzed by immunostaining (red). F Apoptosis was evaluated by flow cytometry analysis. Data are presented as the means ± S.D. Statistical significance is measured using Student’s t test. *: P < 0.05, **: P < 0.01, ***: P < 0.001