Fig. 5
tRF3008A modulates FOXK1 in an AGO-dependent manner. tRF3008A affinity purification-mass spectrometry assay was performed as follows: A Overview of the tRF3008A-protein interactome. B Significant protein enrichment summary with a volcano plot. C GO enrichment analysis was performed to show protein enrichment classification information. D Protein lysates prepared from control and tRF3008A mimetic-transfected HCT116 cells were subjected to Western blot with antibody against AGO and to pull-down of AGO with the tRF3008A probe and oligo (Top); Protein lysates prepared from control and tRF3008A-LNA-transfected HT29 cells were subjected to western blotting, the probe pulled down less AGO in the tRF3008A-LNA-transfected cells than in the control cells. (Bottom). E qRT-PCR analysis of tRF3008A in the HA IP fractions, the pan-Ago IP fractions and Ago2 IP fractions from 293 T cells (left). qRT-PCR analysis of tRF3008A from the pan-Ago and control immunoprecipitation (IP) fractions from HCT116 cells and HT29 cells (Right). F HCT116 cells were co-transfected with tRF3008A mimetic and sh-AGO, and Western blotting analysis of AGO and FOXK1 expression was performed. RNAs isolated from these cells were subjected to real-time PCR. *: P < 0.05, ***: P < 0.001