Fig. 2

RNA-sequencing after downregulating CENP-N. a qRT-PCR was used to verify CENP-N knockdown in 5-8F and CNE-2Z cell lines. b Western blotting was used to verify CENP-N knockdown in 5-8F and CNE-2Z cells. c Heat map showing that the genes altered after CENP-N knockdown were involved in glycolysis, apoptosis, proliferation, and cell cycling. d Bubble plot of KEGG pathway analysis results showing the function of genes altered after CENP-N knockdown. e Bar plot of GO analysis results showing the function of genes altered after CENP-N knockdown. f qRT-PCR was used to verify genes altered after CENP-N knockdown involved in cell glycolysis, apoptosis, proliferation, and cell cycle progression. g Western blotting was used to verify genes altered after CENP-N knockdown involved in glycolysis, apoptosis, proliferation, and cell cycling. The data are shown as the mean ± SD values. * p < 0.05, ** p < 0.01, ***p < 0.001