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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: ZIP10 is a negative determinant for anti-tumor effect of mannose in thyroid cancer by activating phosphate mannose isomerase

Fig. 1

Selective inhibition of thyroid cancer cell growth by mannose. a Thyroid cancer cell lines TPC-1, BCPAP, FTC133, IHH4, 8305C, 8505C and K1 were treated with mannose in dose-dependent manner for 24 h. Cell viability was then evaluated by MTT assay, and IC50 values were calculated using the Reed-Muench method. b The above cells were treated with 20 mM mannose or not for 3 days. MTT assays were then performed to assess the proliferation of these cells in time-dependent manner. c Colony formation assay was performed when these cells were treated with mannose in dose-dependent manner for 7 days (upper panels). Quantitative analysis of colony numbers was presented in the lower panels. d Cells were treated with mannose or not for 24 h, and stained with PI. Cell cycle distributions were then analyzed by the flow cytometry. e, Western blot analysis of cell cycle-related molecules (including p53, cyclin E and cyclin D, pCDK2 and CDK2) in the indicated cells with a 24-h mannose treatment or not. β-actin was used as a loading control. The data were presented as mean ± SD. Statistically significant differences were indicated: *, P < 0.05; **, P < 0.01; ***, P < 0.001

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