Fig. 2

PMI knockdown improves mannose-insensitive thyroid cancer cell responsiveness to mannose. a Western blot analysis of PMI in thyroid cancer cell lines. β-actin was used as a loading control. b Western blot analysis showing PMI knockdown in 8305C, 8505C and K1 cells by two different siRNAs (si-PMI-1 and si-PMI-3). β-actin was used as a loading control. c MTT assays showing the proliferation of the indicated cells treated with 20 mM mannose and control cells. d PMI knockdown-8305C, 8505C and K1 cells, and control cells were treated with mannose in a dose-dependent manner. The representative colony images were shown in the upper panels. Colony numbers were then counted and presented in the lower panels. e PMI knockdown-8305C and 8505C cells were treated with mannose or not for 24 h, and stained with PI. Cell cycle distributions were then analyzed by the flow cytometry. f Western blot analysis of cell cycle-related molecules (including p53, cyclin E and cyclin D, pCDK2 and CDK2) in PMI knockdown-8305C and 8505C after a 24-h mannose treatment. β-actin was used as a loading control. The data were presented as mean ± SD. Statistically significant differences were indicated: **, P < 0.01; ***, P < 0.001