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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: ZIP10 is a negative determinant for anti-tumor effect of mannose in thyroid cancer by activating phosphate mannose isomerase

Fig. 3

The effect of intracellular Zn2+ concentration on enzyme activity of PMI and thyroid cancer cell responsiveness to mannose. a Enzyme activity of PMI in thyroid cancer cells was examined by cysteine carbazole sulfuric acid method. The O.D. values were measured in a spectrophotometer at 560 nm. b The fluorescence was analyzed to measure intracellular Zn2+ concentration after TPEN treatment and FluoZin™-3 incubation by flow cytometer (left panel). Statistical analysis of fluorescence intensity by Student’s t test (right panel). c Enzyme activity of PMI was measured by cysteine carbazole sulfuric acid method after TPEN treatment. d Western blot analysis of PMI in TPEN or DMSO-treated 8305C cells. β-actin was used as a loading control. e The MTT assay showing the effect on mannose on the proliferation in 8305C cells with different treatments. ***, P < 0.001 for TPEN vs. TPEN + mannose. f The effect of mannose on colony formation in 8305C cells with different treatments (upper panel). Colony numbers were counted and presented in the lower panel. g Measurement of intracellular Zn2+ concentration in 8305C cells with the indicated treatments by flow cytometer (left panel). Statistical analysis of fluorescence intensity by Student’s t test (right panel). h Enzyme activity of PMI was tested in 8305C cells with the indicated treatments. The O.D. values were measured in a spectrophotometer at 560 nm. i The MTT assay showing the effect of mannose on the proliferation of 8305C cells with the indicated treatments. The data were presented as mean ± SD. Statistically significant differences were indicated: *, P < 0.05; **, P < 0.01; ***, P < 0.001

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