Fig. 4
ZIP10 increases intracellular Zn2+ concentration and enzyme activity of PMI. a Western blot analysis of ZIP10 in thyroid cancer cell lines. β-actin was used as a loading control. b Western blot analysis showing ZIP10 knockdown in 8305C and 8505C cells by two different siRNAs (si-ZIP10–1 and si-ZIP10–3), and ectopic expression of ZIP10 in TPC-1 and FTC133 cells. β-actin was used as a loading control. c Measurement of intracellular Zn2+ concentration in ZIP10 knockdown-8305C/8505C cells and control cells by flow cytometer (upper panels). Statistical analysis of fluorescence intensity by Student’s t test (lower panels). d Measurement of enzyme activity of PMI in ZIP10 knockdown-8305C/8505C cells and control cells. e Measurement of intracellular Zn2+ concentration in ZIP10 overexpression-TPC-1/FTC133 and control cells by flow cytometer (upper panels). Statistical analysis of fluorescence intensity by Student’s t test (lower panels). f Measurement of enzyme activity of PMI in ZIP10 overexpression-TPC-1/FTC133 and control cells. The data were presented as mean ± SD. Statistically significant differences were indicated: *, P < 0.05; ***, P < 0.001