Fig. 1
From: Dysbiotic microbes and how to find them: a review of microbiome profiling in prostate cancer
A) Prostate tumor microenvironment is shown harboring bacterial, viral, fungal, and archaeal species. 16s amplicon sequencing is useful for profiling e.g. the bacterial taxa, whereas shotgun metagenome (DNA) and metatranscriptome (total RNA) sequencing enables profiling of the entire tumor associated microbiota. B) A general workflow for analysing microbiome data outlining the major steps involved for the three main NGS based methods discussed in this review. QC, quality control. OTU, operational taxonomic unit. ASV, amplicon sequence variants. Figure 1A is inspired by [19]. Image created partly using Biorender.com