Fig. 8
Schwann cell autophagy is inhibited by RO + CQ in vivo. A. CK19 IHC staining of the PANC-1 and BxPC-3 nerve invasion models. T indicates the tumor. Arrow indicates nerve. B. Statistics of the distance (μm) between nerves and tumors in the PANC-1 nerve invasion model (tumor image: Fig. 8A) (* p < 0.05). C. Statistics of the distance (μm) between nerves and tumors in the BxPC-3 nerve invasion model (tumor image: Fig. 8A) (* p < 0.05). D. TEM image of the PANC-1 and BxPC-3 nerve invasion models treated with the control, RO, CQ or RO + CQ. The green arrow indicates autophagosomes. The red arrow indicates apoptosis sign. E. Statistics of the autophagosome number of Schwann cells in the PANC-1 nerve invasion model treated with the control, RO, CQ or RO + CQ (cell image: Fig. 8D) (* p < 0.05). F. Statistics of the autophagosome number of Schwann cells in the BxPC-3 nerve invasion model treated with the control, RO, CQ or RO + CQ (cell image: Fig. 8D) (* p < 0.05). G. LC3 IHC staining of tumors in the PANC-1 nerve invasion model treated with the control, RO, CQ or RO + CQ. Green arrow indicates LC3-positive cancer cells. H. LC3 IHC staining of nerves in the PANC-1 nerve invasion model treated with the control, RO, CQ or RO + CQ. Green arrow indicates LC3-positive Schwann cells. I. LC3 IHC staining of tumors in the BxPC-3 nerve invasion model treated with the control, RO, CQ or RO + CQ. Green arrow indicates LC3-positive cancer cells. J. LC3 IHC staining of nerves in the BxPC-3 nerve invasion model treated with the control, RO, CQ or RO + CQ. Green arrow indicates LC3-positive Schwann cells