Fig. 2

Kaempferol and quercetin inhibit growth, survival and migration of RMS cells. Rh30 (A) and Rh41 (B) cells were treated with different concentrations of kaempferol (K) and quercetin (Q) for 24 h and cell survival was determined and normalized to the DMSO values as outlined in the Materials and methods. Rh30 (C) and Rh41 (D) cells were treated with kaempferol and quercetin for 24 h and whole cell lysates were analyzed by western blot analysis as outlined in the Materials and methods. Rh30 (E) and Rh41 (F) cells were treated with DMSO, kaempferol (K) or quercetin (Q) for 24 h and effects on cell migration were determined in a scratch assay as outlined in the Materials and methods. Results (A, B) are means ± SD for at least 3 determinations and significantly (p < 0.05) decreased growth is indicated (*)