Fig. 3

Kaempferol and quercetin inhibit RMS cell migration and invasion. Rh30 Effects of kaempferol and quercetin (24 h treatment) on invasion of Rh30 (A) and Rh41 (B) cells from medium supplemented with 2.5% fetal bovine serum (FBS) to the medium containing 10% FBS through Matrigel matrix in a Boyden chamber invasion assay were determined and the results were quantified relative to DMSO control. C Rh41 cells were grown as spheroids and effects of kaempferol and quercetin on spheroid cell invasion in matrigel matrix were determined as outlined in the Materials and methods. The relative invasion was quantified in comparison to DMSO treated conditions. Results are expressed as means ± SD for at least 3 determinations and significant (p < 0.05) inhibition is indicated (*)