Fig. 6

Kaempferol and quercetin act as mTOR inhibitors and induce cell detachment in RMS cells. Rh30 (A) and Rh41 (B) cells were treated with kaempferol or quercetin for 24 h and whole cell lysates were analyzed by western blots. A comparable protocol was used to determine effects of kaempferol and quercetin on EMT marker gene products in Rh30 (C) and Rh41 (D) cells. E. Cells were treated with kaempferol and quercetin for 24 h and also transfected with and examined by differential interference contrast imaging as outlined in the Materials and methods