Fig. 2

BMS-1 induces intestinal barrier injury in mice with melanoma. In the B16F10 melanoma model, the mice were intraperitoneally administered with the PD-1/PD-L1 inhibitor BMS-1 (0, 5 and 10 mg/kg) every 2 days for 6 times (n = 10). A Representative images of hematoxylin and eosin (HE) staining of colonic tissues and corresponding quantification analysis. Scale bars, 100 μm. B Representative images of Alcian blue-periodic acid-Schiff (ABPAS) of colonic tissues and corresponding quantification analysis. Scale bars, 100 μm. C Representative images of proliferating cell nuclear antigen (PCNA) and corresponding quantification analysis. Scale bars, 100 μm. D Representative western blot images of Claudin-1, Occludin and ZO-1 in colonic tissues and corresponding quantification analysis. E Representative images of laser Doppler analysis and corresponding quantification analysis. Scale bar, 2 mm. The values are presented as the mean ± standard error of the mean. ^*P < 0.05, ^**P < 0.01 vs. control