Fig. 3
From: Loss of epidermal MCPIP1 is associated with aggressive squamous cell carcinoma
Histological and immunofluorescence analyses of DMBA/TPA-induced Mcpip1eKO papillomas.(a) Representative H&E staining of control and Mcpip1eKO papillomas after 12 weeks of DMBA treatment. The yellow arrowheads indicate cells with heterochromatic nuclei. (b) qRT-PCR analysis of Krt10, Krt14, Krt5 and Krt13 expression. n = 8-9. (c) KRT10 and KRT14 and (d) PCNA immunofluorescence staining in papilloma sections. (e) Representative immunofluorescence staining of α-SMA and CD31 in Mcpip1eKO and control papillomas. The arrowheads indicate double-positive cells (indicating vessel-like structures). (f) qRT-PCR analysis of Vegfa and Lif expression. n = 8-10. The data are shown as the mean ± SD values. Unpaired t-test was used to calculate P-values. *P < 0.05, ****P < 0.0001. kp - keratin pearls. Scale bar: 100 μm